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Tuesday, May 19, 2020 | History

2 edition of involvement of microtubules in Concanavalin A capping in Chinese hamster ovary cells found in the catalog.

involvement of microtubules in Concanavalin A capping in Chinese hamster ovary cells

Jane Esther Aubin

involvement of microtubules in Concanavalin A capping in Chinese hamster ovary cells

by Jane Esther Aubin

  • 296 Want to read
  • 37 Currently reading

Published by s.n.] in [Toronto .
Written in English

    Subjects:
  • Concanavalin A.,
  • Hamsters as laboratory animals,
  • Microtubules

  • Edition Notes

    Statementby Jane Esther Aubin.
    ContributionsToronto, Ont. University.
    The Physical Object
    Paginationxiii, 173 leaves :
    Number of Pages173
    ID Numbers
    Open LibraryOL18922423M

      Microtubules as a target for anticancer drugs. of tubulin subunits that cap and stabilize microtubules. drugs in Chinese hamster ovary cells correlated with changes in the level of Cited by: Microtubules, the third principal component of the cytoskeleton, are rigid hollow rods approximately 25 nm in diameter. Like actin filaments, microtubules are dynamic structures that undergo continual assembly and disassembly within the cell. They function both to determine cell shape and in a variety of cell movements, including some forms of cell locomotion, the intracellular transport of organelles, .

    (). Antigen cap formation in cultured fibroblasts: a reflection of membrane fluidity and of cell motility. (). Binding of concanavalin A to thymic and bursal chicken lymphoid cells. (). Colchicine binding activity in particulate fractions of mouse brain. ().Cited by: Insight into microtubule nucleation from tubulin-capping proteins Valérie Campanaccia, Agathe Urvoasa, n eukaryotic cells, microtubules form different types of arrays to fulfill different functions. For instance, a microtubule aster Microtubules are involved in many key functions of eukaryotic cells Cited by: 1.

    Furthermore, in a temperature-sensitive Chinese hamster ovary cell line (B), the cells had more Glu MTs when the ERC became dispersed at elevated temperature. Microinjecting purified anti-Glu tubulin antibody into B cells at elevated temperature induced the redistribution of . The central paradigm of microtubule biology is that microtubules are stabilized by a ‘GTP cap’, a region at the end of a polymerizing microtubule where GTP hydrolysis has not yet occurred. Direct measurement of the size of this cap in cells was never previously possible. In this issue of Cited by: 6.


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Involvement of microtubules in Concanavalin A capping in Chinese hamster ovary cells by Jane Esther Aubin Download PDF EPUB FB2

The redistribution of fluorescein-labeled concanavalin A (fl-ConA) has been studied in Chinese hamster ovary (CHO) cells. Using double fluorescent labeling with fl-ConA, and rabbit anti-ConA serum with rhodamine-labeled goat anti-rabbit-IgG, we were able to follow receptor mobility in the plane of the membrane and formation of internalized ConA by: The data establish that (i) microtubule disassembly is correlated with enhanced Con A cap formation on normal human lymphocytes; (ii) T and B cells differ significantly from each other and from circulating polymorphonuclear leukocytes with respect to their capping responses after exposure to colchicine; and (iii) there is an abnormal relationship of microtubule assembly to surface topography in the functionally defective SCID by: The relationship between microtubules and concanavalin A surface receptors during concanavalin A capping in primary cultures of rabbit ovarian granulosa cells was examined by electron microscopic.

Colchicine permeation is required for inhibition of concanavalin A capping in Chinese hamster ovary cells Article (PDF Available) in Proceedings of the National Academy of Sciences 72(11) Only in a small fraction of CHO cells are true plasma membrane aggregates, or caps, found.

This predominance of direct pinocytic interiorization over capping was not affected by dibutyryl cAMP or by treatments which can disrupt microtubules, including cold shock or exposure of the cells to anti-mitotic Cited by:   Macrophages activatedin vivo withCorynebacterium parvum and asbestos showed an increased sub-population of cells which capped spontaneously on incubation with fluoresceinated Concanavalin A compared to saline-induced control macrophages.

This capping was unaffected by colchicine but was inhibited by cytochalasin B. The spontaneous capping of activated macrophages Cited by: 3.

Our results suggest that capping may not be a relevant concept for analyzing the behavior of fibroblast plasma membranes. Experimental Procedures Cells The Chinese hamster ovary cell clone CHO-K1 (Kao and Puck, ) was used in all experiments.

Cells were routinely cultured on solid substrate as described previously (Storrie, ).Cited by: Biochimica etBiophvsicaActa () Elseviel BBA Binding of polycation DEAE-dextran to chinese hamster ovary cells induces reversible Ca2+ influx and inhibits capping of concanavalin A acceptor proteins Stuart K.

Calderwood a, Mary Ann Stevenson a Monika Modlinsky b and George M. Hahn b "Joint Center for Radiation Therapy, Harvard Medical School, 50 Binn~T by: 4.

Coss RA, Dewey WC, Bamburg JR () Effects of hyperthermia on dividing Chinese hamster ovary cells and on microtubules in vitro. Cancer Res – PubMed Google Scholar Cress AE, Culver PS, Moon TE, Gerner EW () Correlation between amounts of cellular membrane components and sensitivity to hyperthermia in a variety of mammalian Cited by: Colchicine permeation is required for inhibition of concanavalin A capping in Chinese hamster ovary December Proceedings of the National Academy of Sciences Jane E.

Aubin. Evidence that mi- CTOtubule-depolymermng drugs affect membrane fluidity has already been obtained in other systems, such as the Chinese hamster ovary cell line, showing also that agents like taxol, known to stabilize the microtubules, are able to block the effect ofaniimicrotubule drugs (13).Cited by:   The protein accumulates in plasmodesmata (f), on microtubules in cells at the TE (g), and in inclusion bodies in cells at the LE (h).

i, Infection site caused by TMV encoding MP(Ls1)– by: Centriol distribution during tripolar mitosis in Chinese hamster ovary cells Article (PDF Available) in The Journal of Cell Biology 98(6) July with 48 Reads How we measure 'reads'.

Capping of surface receptors was induced by allo- and hetero-immune sera followed by fluorescein-conjugated antiglobulin serum, or by the plant lectin concanavalin A. Capping could easily be induced in intact cells, but virtually no capping was detected in the nucleus-free cytoplasts.

Interestingly, karyoplasts, which posses cell-membrane components but very little cytoplasm, could be easily induced to cap Cited by: Concanavalin A (ConA), normally a mitogen of T-lymphocytes, was found to be a cell cycle-independent apoptosis-inducing agent in cultured murine macrophage PU cells.

This assertion is based on Cited by:   These data support the hypothesis that these antimitotic agents interact with an intracellular component, probably microtubules, to prevent the directional movement of concanavalin A receptors on the surface membranes of Chinese hamster ovary by: This study provides further evidence for two separate microtubule entities in cycling nontransformed cells: a cytoplasmic microtubule complex and the microtubules of the mitotic spindle.

Although an interchange of tubulin dimers seems to exist between microtubules in the two systems, control of tubule assembly may be under separate by: Alteration of lymphocyte function by quinones through a sulfhydryl-dependent disruption of microtubule assembly intact cells and that activation requires Cat' suggests an important role for microtubules and calmodulin in the activation of guanylate cyclase.

required for inhibition of concanavalin A capping in Chinese hamster ovary cells Cited by: Chinese hamster ovary (CHO) cells maintained in vitro at pH were used to model cells in the acidic environment of tumours.

CHO cells grown at pH develop thermotolerance during 42 degrees C. Microtubules are involved in many key functions of eukaryotic cells, including cell division, intracellular transport, and cell shape. They are hollow tubes made of parallel filaments, themselves formed by the self-assembly of αβ-tubulin molecules.

Whereas microtubules lengthen and shorten from their ends dynamically, their birth, called nucleation, remains poorly by: 1. The crystalloid ER appears in UT-1 cells, a line of Chinese hamster ovary cells that has been chronically starved of cholesterol as a result of growth in the presence of compactin, an inhibitor of reductase.

When cholesterol was provided to UT-1 cells in the form of low density lipoprotein (LDL), the reductase and crystalloid ER were destroyed.beenseen in interphase cells of a variety of cell lines in tissue culture. These include 3T3cells transformed bysimian virus 40 (Fig.

7), the Don line of Chinese hamster lung cells, C6 rat glial cells, and secondary embryonic cells from mouse and chicken.

However, the length and exact shape of the structure as well as the percentage of cells.Detergent-extracted whole mount preparations of human neutrophilic polymorphonuclear leukocytes (neutrophils) were used to study centrosome position and microtubule organization during random, chemokinetic and chemotactic locomotion.

Chemotaxing neutrophils have a polarized external and internal morphology, with centrosomes between the anterior lamellipod and the by: